Human Tumor Pancreas Lysate

Name Human Tumor Pancreas Lysate
Supplier ABBIOTEC
Catalog 450216
Category Tissue Lysate
Prices $175.00
Sizes 100 µg
Applications WB
Species Human
Tissue Pancreas
Disease Tumor
Description Pancreas tumor tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% beta-mercaptoethanol.
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